Identification and Initial Characterization of High Affinity Ligands Binding Specifically to Endothelial Progenitor Cells Using Phage Display Screening

摘要

We report the discovery of novel pepudes that are highly specific for endothelial progenitor cells (EPC) by screening phage display libraries. Our strategy for isolation of ligand peptides is designed to allow using whole cells as an affinity matrix. We show that binding of phage clones is highly specific for EPC since no binding has been observed on human umbilical vein endothelial cells (HUVEC), HL-60 leukemia cells, human neutrophils and lymphocytes. The results from this study contribute to the development of new strategies to be exploited for the design of biomimetic materials for tissue engineering applications.