Enhancement of Mineralization From Bone Marrow Cells on a Nano-Structured Titanium Surface

摘要

The roughness (Ra) of the Ti substrate increased from 0.07 ± 0.01 μn to 0.20 ± 0.03 μm after the nano-scale engineering process in NaF. AFM and SEM confirm the formation of a nano-scale texturedrnsurface and EDS confirmed that there was no fluoride detected on the surface. The Ti control samples incubated in media alone showed no evidence of Ca or P deposits and, therefore, all mineralization occurring in this experiment was cell-mediated.rnFor the bone marrow cells, SEM-EDS results show CaP mineralization at 11 days on both the polished and nano-textured surfaces. Von Kossa staining indicates a strong influence of the nano-textured surface as the coverage of the mineralized nodules on the polished samples is less than the nanoscale surface beginning at 11 days through 21 days (Fig 1). ALP concentration increases at 11 days, in correlation with the mineralized nodules seen with SEM and Von Kossa staining. FT-IR and XRD confirm the presence of a carbonated apatite on both surfaces beginning at 11 days (Fig 2). SEM morphological examination revealed that at earlier time points (7-11 days), cells on the polished surface are flattened and spread out, whereas cells on the nano-textured surface are more spherical in shape. This unique difference in morphology may indicate that cellular activity is dependent upon morphology and therefore, by altering the surface texture, the biological function of bone-forming cells can be enhanced.