The Interplay of Bone-Like Extracellular Matrix and TNF-a Signaling on In Vitro MSC Osteogenic Differentiation

摘要

Mineralized matrix deposition was not adversely affected by exposure to TNF-a for 4-8 days, but was significantly impeded by 16 days of continuous TNF-a. These results simulate the in vivo response, where brief, highly regulated TNF-a signaling stimulates bone regeneration, while prolonged signaling has damaging effects. Thus, this study underscores the exciting potential of PCL/ECM constructs as a more clinically realistic in vitro culture model to facilitate the design of new bone tissue engineering strategies that rationally control inflammation to promote regeneration.