首页> 外文会议>Progress in Electromagnetics Research Symposium(PIERS 2008) >Resonance as a Tool to Transfer Information to Living Systems: The Effect of 7 Hz Calcium Ion Energy Resonance on Human Epithelial Cells (HaCaT) Differentiation
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Resonance as a Tool to Transfer Information to Living Systems: The Effect of 7 Hz Calcium Ion Energy Resonance on Human Epithelial Cells (HaCaT) Differentiation

机译:共振作为将信息传递到生命系统的工具:7 Hz钙离子能量共振对人上皮细胞(HaCaT)分化的影响

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The specific aim of the present work concerns the effectiveness of low frequency electromagnetic fields treatment to modify biochemical properties of human keratinocytes (HaCaT). Cells exposed to a 7 Hz electromagnetic field and 9.2 μT static magnetic field (B_o) and 9 for one hour (twice daily), showed by Scanning Microscopy modification in shape and morphology; these modifications were also associated to different actin distribution revealed by phalloidin fluorescence analysis. Indirect immunofluorescence with fluorescent antibodies against involucrin and β catenin, both differentiation and adhesion markers, revealed an increase in involucrin and β catenin expression, supporting that exposure to electromagnetic field carries keratinocytes to an upper differentiation level.
机译:本工作的具体目标涉及低频电磁场治疗改变人角质形成细胞(HaCaT)的生化特性的有效性。通过扫描显微镜在形状和形态上的修饰显示,细胞暴露于7 Hz电磁场和9.2μT静磁场(B_o),暴露于9小时一小时(每天两次)。这些修饰还与鬼笔环肽荧光分析揭示的不同肌动蛋白分布有关。用针对整合素和分化标志物的抗整合素和β连环蛋白的荧光抗体进行的间接免疫荧光揭示了整合素和β连环蛋白表达的增加,这表明暴露于电磁场可使角质形成细胞达到较高的分化水平。

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