Selection and Breeding for New Strains in Lentinula edodes

摘要

This study described the breeding and selection of two cultivars of Lentinula edodes, K05 and K48. Strain K05 was selected from colchicine-treated dikaryon strain 135, and strain K48 was selected from a di-mon crossing between a colchicine-treated mutant (388B1) from the original monokaryotic strain K303 and a dikaryon strain JL-2. The identification of antagonistic, esterase isozyme, molecular markers etc. revealed that strains K05 and K48 were different from the initial strain 135 and the parent strains K303 and JL-2. The production increase of new strains was notable. Compared with strain 135, the average production of new strain K05 increased by 28. 5%, and compared with the dominant cultivated strain 939, strain K48 increased by 10.7%, and also showed good quality. New strains K05 and K48 are expected to replace the conventional cultivated strains in the future. The results not only meet the requirement to the new strains for mushroom farmers but also offer a new pathway for genetic breeding in Lentinula edodes.