ERK-dependent activation of Spl is required for low-power laser irradiation-induced vascular endothelial cell proliferation

摘要

Here, we report that low-power laser irradiation (LPLI) activates ERK/Spl pathway to upregulate VEGF expression and promote vascular endothelial cell proliferation. We demonstrate for the first time that LPLI enhances DNA-binding activity and transactivation activity of Spl on VEGF promoter. Additionally, ERK translocates from cytoplasm to nucleus following LPLI. Moreover, activated ERK phosphorylates Spl and results in increased EKR-Spl interaction. Selective inhibition of Spl or ERK suppresses the effect of LPLI on the promotion of cell cycle progression and proliferation. These findings provide a novel link between LPLI and angiogenesis, supplying potential therapy strategies for angiogenesis with LPLI.