Effect of Plant Growth Regulators on Callus Induction and Plant Regeneration from Mature Seed of Reed (Phragmites communis Trinius)

摘要

@@1. Objectives In order to develop an efficient, reliable and reproducible tissue culture system for reed (Phragmites communis Trinius) an efficient plant regeneration system via callus induction was established using mature seed as explants. MS medium containing 1 mg/L 2,4-D and 0.5 mg/L BA was optimal for embryogenic callus induction from mature seeds The highest frequency (86.4%) of embryogenic callus was obtained in 1.0 mg/L 2,4-D. The highest plant regeneration frequency (59.6%) was found when the embryogenic calli were subcultured on MS medium supplemented with 100 mg/L myo-inositol, whereas, adding of plant growth regulators was not so promising in this case. Our result would be useful for development of transgenic reed plants.