Proteomic Applications of Drop Coating Deposition Raman Spectroscopy

摘要

Recent studies have demonstrated that the drop coating deposition Raman (DCDR) method may be used to obtain high quality vibrational Raman spectra for the identification and quantitiation of proteins, peptides, glycans, and pharma-ceuticals. Here we demonstrate applications of DCDR to chromatographic detection, and the identification of peptide and protein structural changes induced by phosphorylation, fibrillation and ligand binding. Such results demonstrate the significant promise of normal (as opposed to surface or resonance enhanced) Raman spectroscopy as a proteomic diagnostics, drug screening and chromatographic detection method with exceptional resolution and reproducibility for high performance chemical identification and quantitiation applications.