Development of nanostructured biocompatible materials for chemical and biological sensors

摘要

This research is focused on the fabrication of thin films followed by Surface Enhanced Raman Spectroscopy (SERS) testing of these films for various applications. One technique involves the mixture of nanoparticles with twophoton material to be used as an indicator dye. Another method involved embedding silver nanoparticles in a ceramic nano-membrane. The substrates were characterized by both Atom Force Microscopy (AFM) and Scanning Electron Microscopy (SEM). We applied the nanostructured substrate to measure the SERS spectra of 10sup-6/sup Mol/L Rhodomine 6G(Rh6G), e-coli bacteria and RDX explosive. Our results showed that silver coated ceramic membranes can serve as appropriate substrates to enhance Raman signals. In addition, we demonstrated that the in-house-made colloidal silver can work for enhancement of the Raman spectra for bacteria. We measured the Raman spectra of Rh6G molecules on a substrate absorbed by a nanofluid of silver. We observed several strong Raman bands – 613cm-sup1/sup,768 cmsup-1/sup,1308cmsup-1/sup 1356 cmsup-1/sup,1510cmsup-1/sup, which correspond to Rh6G vibrational modes υsub53/sub,υ6sub5/sub,υ1sub15/sub,υsub117/sub,υsub146/sub respectively, using a ceramic membrane coated by silver. The Raman spectra of Rh6G absorbed by silver nanofluid showed strong enhancement of Raman bands 1175cmsup-1/sup and 1529cmsup-1/sup, 1590 cmsup-1/sup. Those correspond to vibrational frequency modes – υsub103/sub,υsub151/sub,sub152/sub. We also measured the Raman spectra of e-coli bacteria, both absorbed by silver nanofluid, and on nanostructured substrate. In addition, the Fourier Transfer Infrared Spectra (FTIR) of the bacteria was measured.© (2012) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.