Fluctuation Correlation Spectroscopy of Near-field Trapped Nanoparticles

摘要

A new technique, the near-field correlation Spectroscopy (NFCS), is introduced which will be useful for monitoring individual binding events between the biomolecular complex precursors and a functionalized nanoparticle core in a physiological fluid. From jumps in the measured correlation times of single particles trapped in nanoapertures in a metal film, one will be able to determine the rate of binding/dissociation events and, most importantly, the nature of the binding entity, for e.g. discriminate the arrival and binding of a pentamer from a hexamer of protein subunits. This report deals only with the initial steps that are required to implement the new technique.