Background: Cooking and heating with solid fuels produces high levels of household air pollution (HAP) and is a substantial contributor to the disease burden. This study assesses the sources, magnitudes, and chemical compositions of HAP in traditional Eastern Tibetan Plateau households; examines the perturbation of urine metabolome in response to the HAP exposure; and explores how these correlate with cookstove use, fuel type, cooking behaviors, and residents' overall health. Methods: We measured 24-hour personal exposure to PM2.5 (n = 46) and kitchen area black carbon (BC) concentrations (n = 8), using MicroPEM and microAeth, respectively. We also measured particle-bound polycyclic aromatic hydrocarbon (PAH) (n = 12) and metal concentrations (n = 11) during post-analysis of the MicroPEM filter. We conducted household surveys (n = 24) regarding demographics, cookstove usage, fuel type, cooking behavior, and lifestyles. In addition, we collected fasting urine (n= 46) and applied untargeted metabolite profiling to monitor metabolic features and assess associations with measured HAP exposures. These data will be analyzed with bioinformatics approaches-Manhattan plot and Mummichog pathway analysis. Results: Our results reaffirm that burning firewood and yak dung, result in high PM2.5 and BC exposures. The geometric mean (95% CI) concentration was 92.2 (12.5, 682.4) μg/m3 for PM2.5 and the mean ± standard deviation (SD) concentration was 5.8 ± 5.5 μg/m3 for BC, 474.3 ± 584.3 ng/m3 for EPA standard suite of 16 PAHs, respectively. The mean ± SD of concentrations of twenty-five detected metal elements ranged from 0.9 ± 1.1 (Co) to 1585.1 ± 1621.5 ng/m3 (Si). Our statistical analyses illustrate high concentrations of PM2.5, BC, most PAHs and metals are significantly associated with village characteristics and household fuel types. Conclusion: Results from this study provide useful information for understanding the HAP exposure and related health risks in Tibetan communities.
展开▼
机译:背景:烹饪和加热与固体燃料产生高水平的家用空气污染(HAP),是疾病负担的大量贡献者。本研究评估了传统东藏高原户中HAP的源,大幅度和化学组成;响应HAP暴露,检查尿液代谢物的扰动;并探讨这些如何与烹饪用途,燃料类型,烹饪行为和居民的整体健康相关联。方法:使用微量疏水和微读数,我们测量24小时个人接触PM2.5(n = 46)和厨房区域黑碳(Bc)浓度(n = 8)。在微透镜过滤器的后分析期间,我们还测量粒子结合的多环芳族烃(PAH)(n = 12)和金属浓度(n = 11)。我们对人口统计学,烹饪用法使用,燃料类型,烹饪行为和生活方式进行了家庭调查(n = 24)。此外,我们收集了空腹尿液(n = 46)并应用了未标准的代谢物分析,以监测代谢特征,并评估具有测量的HAP曝光的关联。这些数据将分析生物信息学方法 - 曼哈顿绘图和Mummichog途径分析。结果:我们的成果重申燃烧木柴和牦牛粪,导致高PM2.5和BC曝光。对于PM2.5,几何平均值(95%CI)浓度为92.2(12.5,682.4)μg/ m3,平均值±标准偏差(SD)浓度为BC为5.8±5.5μg/ m3,474.3±584.3 ng / m3 EPA标准套件16 PAHS。二十五种检测金属元素的浓度平均值±SD为0.9±1.1(CO)至1585.1±1621.5 ng / m 3(Si)。我们的统计学分析说明了高浓度的PM2.5,BC,大多数PAH和金属与村特征和家庭燃料类型显着相关。结论:本研究的结果为了解藏族社区的HAP暴露和相关健康风险提供了有用的信息。
展开▼