Expression of mineralized tissue associated proteins: Dentin sialoprotein and phosphophoryn in rodent hair follicles

摘要

Objective: In this study, we examined the presence and location of DSP/PP proteins during hair follicle development. Methods: Rat PP proteins were detected using immunohistochemical/immunofiuorescent staining. DSPPP mRNAs were detected by in situ hybridization with riboprobes. LacZ expression was detected in mouse tissues using a DSP-PP promoter-driven LUC in transgenic mice. Results: We found that PP proteins and DSP-PP mRNAs are present in rat hair follicles. We also demonstrate that an 8 kb DSP-PP promoter is able to drive lacZ expression in hair follicles. Conclusion: We have firmly established the presence of DSP/PP in mouse and rat hair follicles by immunohistochemical/immunofiuorescent staining, in situ hybridization with riboprobes and transgenic mice studies. The expression of DSP/PP in hair follicles is the first demonstration that major mineralization proteins likely may also contribute to soft tissue development. This finding opens a new avenue for future investigations into the moleculargenetic management of soft tissue development.