Electrospinning of Emulsion Nanofibers Containing Sorbitan Monooleate and Poly (L-Lactide-co-ε-Caprolactone) for Growing Pig Iliac Endothelium Cells

摘要

The aim of this study was to investigate electrospinning of emulsions to prepare biodegradable nanofibrous mats for being an innovative type of cell-growth scaffolds with potentially controllable drug releasing capabilities. The hypothesis was that the Poly (L-Lactide-co-ε -Caprolactone) P(LLA-CL) nanofibrous mats containing Sorbitan Monooleate (Span-80) could be appropriate scaffolds for growing pig iliac endothelium cells (PIECs). To test the hypothesis, the electrospinning of emulsions made of P(LLA-CL), chloroform, Span-80, and distilled water to prepare P(LLA-CL)/Span-80 nanofibrous mats was systematically investigated. The effects of water content and P(LLA-CL) concentration in the emulsions on the morphologies of the nanofibers were studied. The morphologies of nanofibrous mats were examined by a Scanning Electron Microscopy (SEM); and the performance for being scaffolds was investigated by examination of the viability (anchorage and proliferation) and morphology of PIECs on the nanofibrous mats. The results indicated that the incorporation of Span-80 into P(LLA-CL) nanofibers did not have any negative effect on the growth of PIECs; and the P(LLA-CL)/Span-80 nanofibrous mats could be used as an innovative type of scaffolds with potentially controllable drug releasing capabilities.