Microbial Community Dynamics in Bio-Hydrogen Production Reactor Using Denaturing Gradient Gel Electrophoresis

摘要

To enhance the hydrogen production efficiency in the biological hydrogen producing reactor was one of the important issues for biotechnology of hydrogen production by fermentation. Anaerobic sludge of difference period in the continuous stirred tank reactor (CSTR) was obtained. After DNA extraction of sludge, the V3 region of 16S rDNA was PCR-amplified. The diversity and dynamics of microbial communities were investigated by denaturing gradient electrophoresis gel (DGGE). Population's dynamics were detected every other 7-day and were analyzed. Dominant populations and hydrogen production capacity in each stage are deferent. In the 8th day, dominant population of hydrogen producer changed a lot. The results indicated that a dominant bacterium belongs to family Ethonalogenbacterium Harbin that has a high hydrogen production rate. Cooperative effect of microbial population further suggested that hydrogen production efficiency was determined together by hydrogen producing bacterium and other population.