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Experimental Investigation of Cell Cloning Efficiency after Optical Trapping

机译:光学诱捕后细胞克隆效率的实验研究

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In this paper, chemically etched axicon fiber was investigated for laser trapping of micro-object dispersed in liquid. We fabricated axicon micro lenses on a single-mode bare optical fiber by selective chemical etching technique. The laser beam from fiber axicon microlens was strongly focused and optical forces were sufficient to move a microorganisms and biological cells without physical contact. In our experiments, several different lasers with various wavelengths were used as light sources. From these experimental results, it was found that laser wavelength was very important parameter for cell trapping and laser wavelength should be selected to avoid absorption by cells in order to prevent thermal degradation and damage to the cells.
机译:本文研究了化学蚀刻的轴晶纤维,用于激光分散在液体中的微物体。 我们通过选择性化学蚀刻技术制造在单模裸光纤上的Axicon Micro镜片。 来自光纤轴颈微透镜的激光束强烈聚焦,并且光力足以移动微生物和生物细胞而没有物理接触。 在我们的实验中,使用具有各种波长的几种不同的激光器作为光源。 从这些实验结果来看,发现激光波长对于细胞捕获和激光波长来说,应选择激光波长以避免细胞吸收以防止热劣化和对细胞的损坏。

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