POLYPORUS POLYSACCHARIDE INDUCES THE EXPRESSION OF PEROXISOME PROLIFERATOR- ACTIVIATED RECEPTOR GAMMA IN T24 BLADDER CANCER CELL

摘要

Polyporus polysaccharide (PPS) is the major component of Polyporus umbellatus and has antitumor and immunoregulation functions. It has been used into secondary therapy in post bladder carcinoma patients in traditional Chinese medicine. Peroxisome proliferator-activated receptor gamma (PPARγ) is a member of the nuclear receptor superfamily of ligand-activated transcription factors that has been associated with the inhibition of tumor cell growth. The purpose of this study is to investigate whether PPARγ was involved in the inhibition of T24 bladder cancer cell survival by PPS. In vitro, cell viability was evaluated by using MTT. Peroxisome proliferator-activated receptor gamma (PPARγ) expression in human T24 bladder cancer cells was evaluated by immunocytochemistry, Real-time PCR and Western blot. PPS inhibited the growth of bladder cancer T24 cell. The PPAR γ antagonist biphenol A diglycidyl ether (BADGE) reversed PPS meditated cell growth inhibition.