Dectection of Pesticides Using Microcantilever Immunosensor

摘要

A microcahtilever immunosensor which has been the development for label-free detection of pesticides was exemplified with the ethyl parathion (EP). Sulfhydrylated anti-EP antibody was immobilized on the gold surface of the microcantilever for the measurement of EP. Subsequently, the deflection of the microcantilever corresponding to different EP concentrations was real time monitored by optical lever technique. The activity of sulfhydrylated antibody immobilized on the gold surface of a microcantilever was evaluated by non-competitive enzyme-linked immunosorbent assay (ELISA).The experimental results indicated: although the sulfhydrylation caused loss of 19.9% activities of mAbs, sulfhydrated anti-EP antibody on the gold surface can keep the activity. Deflection of microcantilever is linear to EP concentration from 10～500 ng/mL. The limit of detection is 1.7 ng/mL.