Analysis of Cellulase Activity of Mixed Bacteria and Production of Butanol by Co-Cultured Mixed Bacteria and Clostridium Acetobutylicum ATCC824

摘要

Microbial conversion of lignocellulose to butanol was a fascinating way to provide a renewable energy source. Filter paper degradation rate of soil samples from four different habitants and carboxymethyl cellulase (CMCase) were analyzed. The results showed that the samples from STS (the surface of a tree stump) and DTS (the deep of a tree stump) had higher filter paper degradation rate and CMCase activity. When the soil samples from STS were precultured for 72 h using filter paper as the sole carbon source, and cocultured with Clostridium acetobutylicum ATCC824 for 40 h, the butanol yield was 0.21 g/g filter paper. The strain composition of the samples from STS was researched. Bacterial, actinomycetes, mold and yeasts were filtered and their cellulose degradation ability were analyzed. Bacterial and mold had higher CMCase activity. These results demonstrate that the mix-strains from STS could degrade filtrate paper, the production from which could be used by Clostridium acetobutylicum ATCC824 to produce butanol.