Determination of Ferulic Acid in 'SHI-QUAN-DA-BU' Pills by High Performance Capillary Electrophoresis

摘要

High performance capillary electrophoresis (HPCE) was used in this text to determine the content of ferulic acid in "SHI-QUAN-DA-BU" Pills. Electrophoretic separation conditions: Uncoated fused silica capillary column(75μm×49/58cm), injection height 7.5cm, 30mmol·L~(-1) borax solution, separation voltage 20kV, injection time 15s, detection wavelength 315 nm, experimental temperature 20°C. Linearity was kept in the concentration ranging from 1～31.25 mg·L~(-1) of ferulic acid with correlation coefficient of 0.970. The average recovery was 97.56% and RSD value was 6.4%. The content of ferulic acid was 0.0855 mg/g (n=6).