DIFFERENTIAL EXPRESSION OF HUMAN TELOMERASE REVERSE TRANSCRIFIASE β-SITE REMAINING LTERNATIVE SPLCING VARIANTS (HTERT β+ ASV) IN GASTRIC CANCER AND PRECANCEROUS LESIONS

摘要

Objective:To investigate the changes of hTERT alternative splicing variants pattern in gastric cancer, precancerous lesions and normal gastric mucosa tissue. Methods: Three alternative splicing sites (α, β, γ) were selected using Semi-nested RT-PCR and SYBER Green real-time PCR. Telomerase enzyme activity was evaluated associated with the different hTERT ASVs. Results: The positive rates of β+ ASVs were 11. 1% in normal mucosa, 40. 0% in precancerous lesions and 94. 7% in gastric cancer (P<0. 05). SYBR Green real-time RT-PCR showed that the expression level of β + ASV was 6. 99 times higher in gastric cancer than in precancerous lesions. Further, increased telomerase enzyme activity was only associated with expression of the full-length hTERT isoform. Conclusion: The gene expression patterns of hTERT alternative splicing variants may provide some useful information for diagnosis of gastric cancer and precancerous lesions.