The developmental patterns of concentration and mRNA abundance of growth hormone and insulin-like growth factor-I in serum, liver and longissimus dorsi of suckled crossbred piglets

摘要

A total of thirty (15 male and female, respectively) suckled crossbred piglets (Duroc × Landrace × Yorkshine) were selected and divided into five groups at the ages of d 1, 7, 14, 21, and 28 after brith, respectively. Piglets were weighed, and blood and tissue (liver and longissimus dorsi) samples were collected from a total of 30 piglets at different ages. There were no significant differences (P>0.05) between male and female, but there were significant differences (P<0.05) among ages during the early postnatal development for the concentration and mRNA abundance in serum, liver and longissimus dorsi (LD) of suckled crossbred piglets growth hormone and insulin-like growth factor-I (IGF-I). There were the different dvelopment curve pattern (linear, quadratic, cubic, or quartic) of age effects (P<0.05) on the concentration and mRNA expression of suckled crossbred piglets GH and IGF-I during the early postnatal development from 1 to 28 day of ages. Pearson analysis showed serum GH concentration was correlated with serum IGF-I concentration, IGF-I mRNA abundance of LD, BW, and the relative body weight of LD. Serum IGF-I concentration was correlated with liver IGF-I concentration, IGF-I mRNA abundance of LD, and BW. Liver IGF-I concentration was correlated with GHR mRNA abundance of LD, IGF-I mRNA abundance of liver and LD, IGF-IR mRNA abundance of LD, BW, and the relative body weight of LD. LD IGF-I concentration was correlated with IGF-I mRNA abundance of liver and LD, IGF-IR mRNA abundance of LD, and BW. Liver IGF-I mRNA abundance was correlated with GHR mRNA abundance of liver, IGF-I mRNA abundance of LD, BW, and the relative body weight of LD. LD IGF-I mRNA abundance was correlated with GHR mRNA abundance of liver, IGF-IR mRNA abundance of LD, BW, and the relative body weight of LD, respectively.