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Simple and Sensitive Protein Detection System using UV LEDs and Liquid Core Waveguides

机译:使用UV LED和液体芯波导的简单敏感的蛋白质检测系统

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Protein concentrations are traditionally analyzed in the ultraviolet at 280nm using laboratory spectrophotometers. Recently, AlGaN/GaN deep ultraviolet Light Emitting Diodes (LED) became available in the 250 nm to 350 nm wavelength region. An inexpensive fiber optic detection system based on these UV LEDs and photodiodes has been developed. Measurement stability, noise and drift will be discussed. To improve sensitivity of the detection system, microliter volume liquid core waveguide sampling cells with 10 cm and 50 cm pathlength were evaluated. With a continuous flow setup, protein concentrations as low as 1 μg/mL and 0.2 μg/mL were detectable in a 10 cm and a 50 cm pathlength cell respectively, based on a 10 mAU absorbance signal and a signal to noise ratio greater than 10.
机译:使用实验室分光光度计在280nm处在紫外线中分析蛋白质浓度。最近,AlGaN / GaN深紫外光发光二极管(LED)在250nm至350nm波长区域中可用。已经开发出基于这些UV LED和光电二极管的廉价光纤检测系统。将讨论测量稳定性,噪声和漂移。为了提高检测系统的灵敏度,评估具有10cm和50cm的流程的微升体积液体核心采样电池。通过连续流量设置,基于10 mau吸光度信号分别在10cm和50cm的路径和50cm的路径和50cm的路径和50cm的路径和0.2μg/ ml中检测到低至1μg/ ml的蛋白质浓度,并且噪声比大于10 。

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