Investigation on dynamic optical clearing effect of skin tissue under topical application of hyperosmotic agents studied with FT-IR imaging as an analytical tool

摘要

Currently, to enhance light penetration into tissue and improve imaging performance for high-resolution imaging techniques, optical clearing of biological tissue is normally accomplished by the impregnation of bio-tissues with the hyperosmotically active agents. The results from previous studies appear encouraging but there is, as yet, a relatively poor understanding of the mechanisms involved. By using FT-IR imaging technique, we perform a preliminary investigation on dynamic processes occurring in hyperosmotic agents penetrating into skin tissue in vitro. The sequential collection of images in time series provides an opportunity to assess the movement of glycerol and DMSO beneath the surface of skin tissue over time. The results presented show the potential of FT-IR imaging as an analytical tool for the study on dynamic optical clearing effect when the bio-tissue is impregnated by hyperosmotic agents. A further exploration of FT-IR imaging in this regard would be desirable to quantify hyperosmotic agents used for optical clearing of different biological tissues.