Development of Microfluidic Device for Heterogeneous Immunoassay on Polystyrene Platform with Electroosmotic Control

摘要

We have proposed an ultra-fast method for the determination of antibody concentration in an analyte. The method is based on a short pulse introduction of the analyte into the flow-through microcapillary. The mathematical model of transport and kinetic processes in a capillary microchip for heterogeneous immunoassay (IgG -Protein A) and results of simulations are described. Effects of surface heterogeneities at the immobilized antigen and the effects of kinetic, geometric, and operational parameters are studied. It is demonstrated that 1 sec pulse of the analyte is long enough to qualitatively determine antibody concentration in a wide range of the values of model parameters. The corresponding experimental microchip on the polystyrene matrix was constructed by the wire imprinting method. The measured value of the electroosmotic mobility coefficient which characterizes flow through the microchip is μ{sub}(eo) = 2.83±0.11×10{sup}(-8) m{sup}2V{sup}(-1)s{sup}(-1).