3-D droplet transportation of Electro Wetting On Dielectric (EWOD) actuation with flexible substrate is proposed. Instead of glass substrate, a polymer film is taken to fabricate the microfluidic device for the droplet demonstrating out of plane motions. Two kinds of tests, tilted test and curved test, are performed, and a model of minimum required actuation voltage is presented, and compared with the results of tilted test. The current model matches experiment data much better than the conventional ones. In the curved test, the droplet successfully transports from bottom to the top.
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