Detection and Quantitation of Carbohydrates in the Murine Gastrointestinal Tract Following Antibiotic Treatment and During Clostridium Difficile Infection

摘要

A sample preparation method was established using both liquid and solid phase extraction steps to clean up the complex matrices in these murine gut samples. A HILIC LC-MS/MS method was developed in negative ESI mode with isotope dilution technique for quantitative analysis of mannitol and sorbitol from murine gut in three biological conditions in C57BL/6 mice. The levels of mannitol and sorbitol were about the same, at about 1200 ppb, in the non-treated condition. There was 149 % increase in sorbitol concentration in antibiotic-treated mouse gut than in non-treated ones, while a 48% decrease in mannitol concentration was observed. CDI (infected) mice showed that both alditol concentrations decreased by more than 30% and 70% respectively, compared to non-treated mice Sorbitol contributed to the increased total carbohydrates following antibiotic treatment, confirming the findings of untargeted work.