Quantitative Phosphoproteomic Analysis of Cellular Defenses using Stable Isotope-Coded ERLIC/IMAC Combined with LC/MS/MS and LC/MS~(E)

摘要

1. We have successfully isotope-coded A. thaliana proteins at a 99percent-incorporation level at 46 days, the maximum that can be achieve based on the ~(15)N source. 2. The Mascot search results tend to favor ~(14)N-peptide identification over ~(15)N-peptide by a factor of 2 to 1. 3. The automated quantification by Mascot Distiller provided a useful tool for fractionation alignment and peak integration even though some levels of manual examination may be required to overcome modeling errors due to sample complexity.