Comprehensive study of significant nanoLC-MS/MS parameters for proteomic analysis on Q-TOF mass spectrometers thanks to design of experiment

摘要

The results obtained in this study allow a comprehensive adjustment of LC-MS/MS parameters to have an optimized method for proteomic studies. Complex sample (60 to 135 proteins by run) can be analyzed with an improvement of 30percent in the number of unique peptides compare to a non optimized method. Using design of experiments reveals to be an efficient tool to better understand the mechanisms of Data Dependant Acquisition on LC-MS/MS. The resulting method improvement allows identifying more peptides for better protein sequence coverage in a single run.