Acid mine drainage sediment: a reservoir for isolation bioleaching microbes

摘要

Structure and diversity of microbial communities in acid mine drainage(AMD) are well studied in past decades, however, the microbial community structure of AMD sediment and its relationship between sediment and drainage is still unknown. In this study, four samples which include two mine drainages and two sediments were taken from two typical sites of Dexing copper mine in southeast of China. Whole genome of microbial community in AMD and its sediment was extracted, purified, amplified, labeled and hybridized with GeoChip 2.0, a functional gene array covering genes involved in N, C, P, and S cycling, metal resistance and contaminant degradation, to analyze the microbial community. In total, 28 to 1875 functional genes were detected from 4 samples. Sediment nearby the mine drainage may play important role in microbial geochemical process since more functional genes and higher diversity were detected from sediment than AMD. Results indicated that GeoChip 2.0 is a good tool to monitor the AMD's functional genes and AMD sediment may play an important role in generating AMD and for bioleaching.