The N K edge NEXAFS showed greater intensity of the pi~* peaks at glancing incidence compared to normal incidence. The pi~* peaks in the nitrogen region are likely coupling with the aromatic rings of the bases themselves. The increase in polarization dependence after the backfill indicates that the plane of these DNA base aromatic groups are, on average, oriented more parallel to the surface than the bases on the non-backfilled surface. This can be interpreted as the DNA backbone oriented more perpendicular to the surface when there is the backfilled molecule present, which may improve hybridization efficiency of this surface. This change in orientation may be due to the backfill preventing amine groups present on the bases from interacting with the gold surface.
展开▼
机译：与正常发病率相比，N k Edge NexaFs显示出渗透发生率下的PI〜*峰的强度。氮区域中的PI〜*峰值可能与底座本身的芳环耦合。回填后偏振依赖性的增加表明，这些DNA基础芳族基团的平均平均，平均，比未回填表面上的基部更平行。当存在填充分子存在时，这可以将其解释为更垂直于表面的DNA主干，这可以提高该表面的杂交效率。这种取向变化可能是由于回填，防止基座上存在的胺基与金表面相互作用。
展开▼
