Correlation Of Electrical Impedance, Cytokine Expression And Histology At The Biosensor-brain Tissue Interface

摘要

The in vivo impedance, cytokine expression and histological data taken together are consistent with the well-established phases of wound healing, and where the brain foreign body response is responsible for an increase in electrical impedance over the first weeks after device implantation. Intense GFAP~+ staining is suggestive that the reactive astroglial layer may be a mechanism for increasing impedance as a diffusive barrier is formed. The correlation between ED-1~+ staining intensity, levels of inflammation-associated cytokines and reduction in neurofilament density next to the HFM suggests that a chronic inflammatory response to the implanted device may be responsible for loss of single unit recording ability in chronic recording preparations. This HFM device has been demonstrated as a novel tool for characterizing temporal changes at the biosensor-brain tissue interface, allowing multiple data types to be extracted from a single animal experiment.