Application of cell engineering of herbal Medicine treating bone resorption of osteoclasts

摘要

To investigate application of cell engineering in herbal Medicine treating bone resorption. Study design: the bone marrow cells were isolated from 5 week-old SD rats and induced by 1,25(OH){sub}2D{sub}3. Firstly, Morphological assay, cells were fixed, stained by tartrate-resistant acid phosphatase (TRAP) and observed with the fluorescence micrograph. F-actin rings of OCLs were labled using Bodipy FL Phallacidin and PI, and observed with the laser scanning confocal microscope, then also showed the ultrastructure of them with the scanning electron microscope. Secondly, bone pits were observed after bone slices were stained by paraosaniline hydrochloride. Thirdly, the number of TRAP (+) cells with above two nuclei was counted, and TRAP activity of OCLs was examined with TRAP kit. In addition, effects of Traditional Chinese Medicine, such as Oral Liquid of Guiluerxian, on the differentiation and function of OCLs were observed. Results indicated that application of cell engineering made it possible to observe vividly the morphology of osteoclasts, study its function and mechanism underlying bone resorption.