Molecular characterization of partial amphiploids from Triticum durum x tetraploid Thinopyrum elongatum as novel sources of resistance to wheat Fusarium head blight.

摘要

Three amphiploids (2n=6x=42), namely 8801, 8802 and 8803 derived from crossing Triticum durum x tetraploid Thinopyrum elongatum, were screened for Furarium Head Blight resistance. The diseases did not spread beyond the inoculated spikelet in any of the tested plants. Genomic in situ hybridization (GISH) using genomic DNA of tetraploid Th. elongatum as a probe, indicated that they all contained 14 alien chromosomes from tetraploid Th. elongatum. There were no gross chromosomal structure changes in allof the three amphiploids. High molecular weight (HMW) glutenin and gliadin analysis showed that 8802 and 8803 had the same protein bands as wheat. In addition, the three lines contained a specific HMW band come from tetraploid Th. elongatum. These new amphiploids offer an excellent means of introducing Fusarium resistance into durum wheat.