A novel approach to characterize Haemophilus parasuis field isolates using a PCR assay for detecting a neuraminidase gene and using a spot test for estimating the neuraminidase activity expressed by the isolate

摘要

Haemophilus parasuis, which was once considered as the causative agent of a sporadic disease of young pigs compromised by stress, has become one of the most important bacterial pathogens in immune-naive pig herds under todays modern intensive pig raising systems. Innovative new production technologies such as segregated early weaning and all-in/all-out pig flow in high health pig herds and the emergence of new respiratory diseases have been suggested to be associated with an increase in the prevalence and severity of this disease.