Brewers' spent grain (BSG) and spent cell yeast (SYC) are the wastes from mashing and fermentation process of beer, respectively. The compositions of BSG were hemicellulose, cellulose and lignin at 24.49±0.91 %, 20.86±0.60 % and 9.62±0.40 %, respectively. While those of SYC were protein, carbohydrate and lipids at 46.97±0.07%, 21.32±0.08% and 5.73±0.57%, respectively. The BSC and SYC loadings were varied at 5 - 24%. The optimal BSC loading was 15% which gave the maximum total sugar concentration of 33.76±0.67 g/L and nitrogen of 0.75±0.06 g/L while the optimal SYC loading was 24% which gave the maximum total sugar concentration of 62.12±0.15 g/L and nitrogen of 7.03±0.01 g/L. The total sugar concentration in the hydrolysate was adjusted to 40 g/L and used as nutrient sources for cultivation of three oleaginous yeasts, Trichosporonoides spathulata JU4-57, Rhodotorula mucilaginosa G43 and Yarrowia lipolytica TISTR 5151. The yeasts could grow and produce lipids at 1.10±0.03 g/L, 0.51±0.03 g/L and 0.83±0.08 g/L, respectively when using BSG hydrolysate and 1.07±0.03 g/L, 0.73±0.06 g/L and 1.13±0.06 g/L, respectively when using SYC hydrolysate.
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机译：啤酒厂的废谷物（BSG）和废细胞酵母（SYC）分别是啤酒捣碎和发酵过程中产生的废物。 BSG的组成分别是半纤维素，纤维素和木质素，分别为24.49±0.91％，20.86±0.60％和9.62±0.40％。 SYC的蛋白质，碳水化合物和脂质分别为46.97±0.07％，21.32±0.08％和5.73±0.57％。 BSC和SYC的负载变化为5-24％。最佳BSC负载量为15％，最大总糖浓度为33.76±0.67 g / L，氮为0.75±0.06 g / L，而SYC最佳负载量为24％，最大总糖浓度为62.12±0.15 g / L，氮为7.03±0.01 g / L。将水解产物中的总糖浓度调节至40 g / L，并用作培养三种含油酵母，Trichosporonoides spathulata JU4-57，Rhodotorula mucilaginosa G43和解脂耶氏酵母TISTR 5151的营养来源。酵母可以在1.10处生长并产生脂质。使用BSG水解物时分别为±0.03 g / L，0.51±0.03 g / L和0.83±0.08 g / L，使用BSG水解物时分别为1.07±0.03 g / L，0.73±0.06 g / L和1.13±0.06 g / L SYC水解物。
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