Correlation of metabolites in saliva and In Vivo tissue of oral cancer patients based on Fluorescence spectral deconvolution

摘要

In vivo Native Fluorescence spectroscopic characterization of oral tissue and saliva of same group of normal, and patientswith oral squamous cell carcinoma conditions were studied at 350 nm excitation. The measured fluorescence emissionspectra exhibit broad emission with peaks due to NADH, FAD and Porphyrin. To resolve the emission from individualfluorophores, the measured fluorescence spectra were subjected to spectral deconvolution. Further, the variations inrelative distribution, peak shifts and spectral broadening were analyzed with respect to the fluorophores, NADH, FAD,and porphyrin. The changes in the above photophysical characteristics of various native fluorophores between normal andcancer group in both tissue and saliva confirms that there is a significant molecular level changes during the transformationof normal into cancer. The extracted spectral signatures of tissues and saliva were also subjected to linear discriminantanalysis and the diagnostic accuracy between tissue and saliva were compared.