STUDY ON MID-IR SPECTROSCOPY ON WHOLE BLOOD SAMPLES FOR HUMAN GLUCOSE QUANTIFICATION APPLICATIONS

摘要

The importance of a constant blood glucose concentration monitoring in order to keep a regular control for diabetic patients, had been established from the medical approach. Several studies accept the necessity of exploring alternatives for the traditional digital glucometer, given the pain and discomfort related to this technique, which can lead to a compromised control of the disease. Numerous efforts based on the application of IR spectroscopy for non-invasive glucose quantification had been done with favorable, yet not conclusive results, given in part from the research protocols defined, which had not considered the compounds involved in the glucose regulation mechanism, it's known that this substances have an important role from both the biochemical and optical perspective. Therefore it's necessary to apply an interdisciplinary study based on the properties of the glucose in the human body, to understand the interaction between this substance, its surroundings and light from the mid-IR region. From our results, the window of interest for blood glucose is in the spectral range of 1150-950cm~(-1). This study proposes a comprehensive approach of glucose quantification by means of mid-IR absorption spectroscopy, considering important biochemical, physiological and optical properties, we also propose the use of chemometric tools for the analysis of the bio-optical signals. The results of this work would help to define the right parameters aiming to obtain an optical glucose quantification system and protocol.