Intrinsic Fluorescence Spectra of Tryptophan, Tyrosine and Phenyloalanine

摘要

This paper presents the intrinsic fluorescence spectra characteristics of tryptophan, tyrosine and phenylalanine measured with 3D fluorescence spectrophotometer. Two strong fluorescence peaks of tryptophan locate at λex/λem=230/348nm and 280/348nm, three strong fluorescence peaks of tyrosine locate at λex/λem=202/304nm, λex/λem=220/304nm and 275/304nm, and two fluorescence peaks of phenyloalanine locate at λex/λem=210/280nm and λex/λem=260/280nm were found. The linear relationship of fluorescence intensity to solution concentration were also observed in condition of low solution concentration. The influence of pH of solution to the fluorescence intensity was also measured and discussed.