Expression of the Gene Lg-ATF1 Encoding Alcohol Acetyltransferases from Brewery Lager Yeast in Chinese Rice Wine Yeast

摘要

Brewery lager yeast has a homologous gene of ATF1 called Lg-ATF1 which encodes alcohol acetyltransferase, the most important enzyme for acetate esters synthesis. But only ATF1 gene exists in Chinese rice wine yeast. In this study, the Lg-ATF1 gene was cloned from brewery lager yeast S-6, and successfully expressed in the IAH1 site of Chinese rice wine yeast genome, under the regulation of the yeast phosphoglycerate kinase I gene promoter (PGK1_P) and terminator (PGK1_T). Subsequently, the esters formation of the recombinant and control strain was monitored by gas chromatography. The results indicated that the expression of the Lg-ATF1 gene could improve the level of ethyl acetate and isoamyl acetate in Chinese rice wine yeast. The concentration of ethyl acetate and isoamyl acetate produced by the recombinant increased to 70.91 and 8.66 mg L~(-1), respectively. The observations can lead to the development of new strains of Chinese rice wine.