The influence of paddy soil bacterial diversity affected by heavy metals contamination of Dabaoshan Mine

摘要

We studied the paddy soil affected by acid drainage of Dabaoshan polymetallic ore in north Guangdong. Using real-time PCR and PCR-DGGE technique, we explored the influence of the soil bacterial community diversity at different degrees of heavy metals contamination of heavy metal and available content. The results showed that: the number of bacterial, paddy soil bacterial diversity, Shannon-Wiener index and evenness index were significantly changed after Cd, Zn, Pb and Cu pollution. The number of bacterial at upstream Liangqiao was significantly lower than the middle Shuilouxia and downstream Upper Dam, reduced by 3.82×107(cells/g soil) and 2.52×109(cells/g soil), respectively. The bacterial diversity, Shannon-Wiener index and evenness index represented as following: the upstream Liangqiao< middle Shuilouxia< downstream the Upper Dam. UPGMC cluster analysis showed that the paddy soil bacterial community structure was significantly affected by heavy metals contamination. Therefore, different levels of heavy metals contamination significantly changed the bacterial richness of the soil environment flora and affected the dominant bacterial, thus, changed the diversity of agricultural soil microbial community structure.