Molecular Cloning of the cDNA of LPL on Goose and the Phylogenetic Relationship in the Lipase Superfamily

摘要

Lipoprotein lipase (LPL) of Tianfu meat goose was cloned and sequenced using a RT-PCR approach. The nucleotide sequence covered 468 bp with an open reading frame of 155 amino acids. Alignment analysis indicated that the nucleotide sequences are highly conserved to other species studied including chicken, domestic guinea pig, house mouse, Norway rat, cattle, cow and goat, and the homologies are 92.38%, 73.32%, 73.90%, 75.20%, 72.95%, 71.61% and 73.06%, respectively. Topology prediction showed goose LPL including two N-linked glycosylation site Asn187 and Asn215; one catalytic triad (Asp183 and His268); one conserved heparin binding site (Arg134 to Arg137, (RKNR)); eight cysteine residues (Cys68 and Cys94; Cys119 and Cys138; Cys130 and Cys133; Cys285 and Cys306) that are involved in four disulfide bridges; and one lipid-binding site (Trp256 and Trp257 (WW)). Furthermore, molecular phylogeny analyses have shown that LPL protein families were divided into two main evolution branches, one was the LPL-EL-HL branch, and the other was the PL-PLA1 branch.