A MICROARRAY TO ANALYZE METHYLATION PATTERNS OF E-CAD GENE 5'-CpG ISLANDS

摘要

Aberrant methylation on the CpG sites in the promoter regions of the tumor suppressor gene has been shown to associate closely with carcinogenesis. Several studies suggest that the E-cadherin promoter frequently undergoes hypermethylation in multiform carcinogenesis. We have selected a 260 bp segment of a promoter of the E-cad gene as the target sequence and desighed a set of oligonucleotide probes to assemble a methylation specific oligonucleotide microarray to discriminate the methylation patterns of several adjacent CpG sites. We have established five calibration curves of the corresponding probes with fully methylated and unmethylated allele served as negative and positive target. And sequently this microarray assay has been simply applied for mapping methylation patterns in multiple CpG loci of E-cad gene in normal and acute leukemic samples. The methylation oligonucleotide microarray can be a useful and powerful tool to map methylation patterns in multiple CpG island sites.