Saikosaponin-d Prevents the Progression of Mesangioproliferative Glomerulonephritis in Rats

摘要

Aims: the preventive effects of Saikosaponin-d (Ssd) on the progression of mesangioproliferative glomerulonephritis, induced by intravenous infection of anti-Thyl monoclonal antibody (mAb) 1-22-3 into uninephrectomized rats, were evaluated. Methods: eighteen rats were divided into three groups of six. One group was treated with phosphate buffered saline (PBS) as a control. The other two groups were treated with Ssd 1.8 mg/kg body weight (BW)/day or 0.6 mg/kg BW/day respectively. Urine samples were collected from the rats in metabolic cages on days 1,4, 8, 14 and 21 after the injection of mAb 1-22-3. Systolic BP (SBP) was tested on days 1, 5, 10, 14 and 18 after the injection. All rats were sacrificed 21 days after the injection of mAb 1-22-3 for blood biochemistry, RNA preparation from isolated glomeruli, and immunofluorescent and light microscopy of the kidney. Results: Ssd suppressed proteinuria, extracellular matrix expansion, crescentic formation as well as inflammatory cell infiltration recognized by mAb OX8, EDI and TRPM3. Especially, Ssd was shown to have highly significant suppressive effect on CD8+ T cells infiltration into the periglomerular and tubulointerstitial areas, as shown in Table 1 and on expression of a -SMA along Bowman's capsule as shown in Figures 7E and 7F. Conclusion: Saikosapoin-d prevented the progression of mesangioproliferative glomerulonephritis in rats, which might be closely linked to the markedly significant suppressive effects of Ssd on CD8+ T cells infiltration into the periglomerular and tubulointerstitial areas, as well as on the expression of a -SMA along Bowman's capsule.