Preparation of monoclonal antibodies to cryIA insecticidal crystal protein and application to determinate toxin level in Bt cotton

摘要

In this paper, The spore/crystal complex was prepared from Bacillus thuringiensis var. kurstaki HD-1 and HD-73 during sporulation. The parasporal crystal was isolated from the spore crystal/complex by liquid two phased methods. CryIA and CryIA(c) were precipitated from parasporal crystal on PI4.4. The 130-140 kDa molecular weight protein was separated with wodium-dodecy-sulfate polyacrylamide gel (SDS-PAGE) electrophoresis.