Response of U-87MG in nude rats in photodynamic therapy

摘要

Abstract: The response of a human glioma cell line (U-87MG) and normal rat brain to photodynamic therapy (PDT) was investigated in athymic nude rats. U-87MG was implanted in the right hemisphere, and allowed to grow for 17 days. The animals were injected IV with Photofrin$+R$/ (12.5 mg/kg) 48 h prior to PDT of both normal and tumored brain (35 to 280 J/cm$+2$/, 100 mW/cm$+2$/). The results were compared with that obtained under identical conditions with 9L gliosarcoma implanted in Fisher 344 rats. No tumor or normal tissue response was evident in the Nude rats at 35 J/cm$+2$/. Tumor and normal tissue response was evident at incident optical energy dose levels of 140 and 280 J/cm$+2$/. The response of the U-87MG tumor is consistent with that observed with the 9L gliosarcoma, however, the response in the nude rat normal brain was less than that observed in the Fisher 344 animals. These data indicate: (1) a possible contribution of the cellular immune system to the PDT reaction, (2) the human glioma xenograft model may contribute important insights into tumor and normal brain PDT responses. !0