A Substrate-Independent Smart Antibacterial Surface for the Rapid and Effective Killing and Release of Adherent Bacteria

摘要

A universal platform with the capability both to kill attached bacteria efficiently and rapidly to remove dead bacteria and debris to maintain antibacterial properties long-term has been developed. The fabrication process involves the sequential deposition of a GNPL as a photothermal agent and PTLF as a "sacrificial" layer. A typical surface prepared on gold substrate was able to kill >99% of attached bacteria under NIR laser irradiation. In addition, subsequent incubation of the surface in Vitamin C solution removed the killed bacteria and regenerated bactericidal activity. The surface could be used and regenerated in this way through multiple cycles. The method was applied to various substrate materials having a range of surface properties. These antibiotic-free, easily cleaned and regenerated hybrid films offer a viable alternative for the killing and removal of adherent bacteria (particularly multidrug resistant bacteria) on the surfaces of medical devices for in vitro applications.