Co-Culture of Osteoblasts and Endothelial Cells in a Chitosan/ GDP Scaffold to Improve Angiogenesis and Osteogenesis for the Treatment of Critical Size Bone Defects

摘要

These results provide a foundation for the successful co-culture of pre-osteoblasts and endothelial cells in the chitosan/guanosine diphosphate sponge. The co-culture in the multi-layer formation at ratio 1:1 of both cell types provided an environment in which cellular activity was increased compared to mono-cultures. We expect this configuration will be most appropriate to achieve the desired properties, allow for cross-talk between the two cell types and more closely mimic the native bone matrix. More research needs to be conducted regarding the viability of the co-culture in this scaffold in an in-vivo environment.