We have shown that combinations of multiple materials within FRESH prints can produce tissue architectures that resist cell-driven deformation without sacrificing native ECM biochemistry. Resulting constructs can culture epithelial cells into complex tubular monolayers mimicking the division between lumen and stroma found in vivo. We also demonstrate the ability to create and preserve (through handling/imaging) complete, to-scale, high-fidelity ductal epithelial trees modeled on tomographic data from mouse mammary glands.
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