Galectin-enzyme fusion nanoassemblies for prolonged injection site biocatalysis via local glycan binding

摘要

Endowing enzymes with carbohydrate-binding properties via fusion to Gal3 provides prolonged catalytic activity at an injection site. The duration of localized catalytic activity is dependent on the number of Gal33 units, which can be tuned by engineering enzyme-Gal3 fusion proteins to self-assemble into multimeric nanomedicines. We envision that this simple and general approach can address long-standing challenges in therapeutic enzyme delivery, including the need to minimize off-site effects and prevent rapid clearance.