Spatiotemporally controlled transgene expression in hydroxyapatite-doped fibrin scaffolds using high intensity focused ultrasound

摘要

Doping fibrin scaffolds with HA increased the acoustic attenuation and temperature elevation when exposed to HIFU. Thus transgene activation occurred at lower HIFU exposures than in fibrin only scaffolds. HA-doping did not impact cell proliferation. Transgene activation occurred when C3H-fLuc cells were exposed to HIFU generating sufficient hyperthermia to activate the gene switch. The in vivo results indicate that HIFU can spatiotemporally-control transgene activation. Also, C3H-fLuc cells could be reactivated one week after implantation. Future studies will investigate the spatiotemporally-controlled activation of VEGF and BMP2 transgenes using HIFU.